Live Cell Imaging of GFP-tagged MGSP1 During Mitosis

HEK cells with stably transfected MGSP1 (GFP) exhibiting localization to mitotic spindle during metaphase. Images acquired with an LSM 880 confocal microscope (Zeiss) at indicated timepoints where each frame shows approximately 23µm x 23µm. Laser power for the 488nm (MGSP1) and 405nm (DAPI) lasers were 0.3% and 10%, respectively.